Journal: Cell reports

Article Title: Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection

doi: 10.1016/j.celrep.2022.111817

Figure Lengend Snippet:

Article Snippet: pmU6 ST , This work, Addgene #188713 , Cat# 188713.

Techniques: Control, Virus, Recombinant, Cell Culture, Adjuvant, Gas Phase Electrophoretic Molecular Mobility Analysis, Enzyme-linked Immunosorbent Assay, Derivative Assay, Software, Real-time Polymerase Chain Reaction

Journal: Oncotarget

Article Title: Targeting Krebs-cycle-deficient renal cell carcinoma with Poly ADP-ribose polymerase inhibitors and low-dose alkylating chemotherapy.

doi: 10.18632/oncotarget.28273

Figure Lengend Snippet: Figure 1: Fh1- and Sdhb-deficient cells accumulate fumarate and succinate respectively and display decreased oxygen consumption rate. (A) Western blot confirming CRISPR/Cas9 mediated KO of Sdhb (left panel) and Fh (right panel). GAPDH was used as a loading control. (B) Functional validation of succinate (left panel) and fumarate (right panel) levels by LC-MS in isogenic cell lines (n = 3). (C) Seahorse assay showing altered OCR in Fh1-KO (top panel) and Sdhb-KO (bottom panel). (D) Proliferation rates of mutant vs. parental cells (n = 3). Error bars represent means ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05.

Article Snippet: CRISPR/Cas9 genomic editing was performed in RENCA cells using expression of both Cas9 (Addgene #43861) and a gRNA plasmid construct pmU6-gRNA (Addgene #53187) gifted by Dr. Keith Joung [36] and Dr. Charles Gersbach respectively [37].

Techniques: Western Blot, CRISPR, Control, Functional Assay, Biomarker Discovery, Liquid Chromatography with Mass Spectroscopy, Mutagenesis